ESTABLISHMENT OF THE NEW SUBGROUP 16SrI-S (rr-rp) tuf-H BELONGING TO ‘Ca. PHYTOPLASMA ASTERIS’ IN WILD AND CULTIVATED PLANTS IN ARGENTINA

L. Torres, E. Galdeano, F. Fernandez, N. Meneguzzi, L. Conci

Abstract


Argentinian catharanthus little leaf phytoplasma (ACLL) was found naturally infecting Daucus carota L. (carrot), Catharanthus roseus (LG Don) (periwinkle) and wild Matricaria chamomilla L. (chamomile). All infected plants showed symptoms of yellowing, little leaf and internodes shortening. Flower scape distortion, malformations of umbels and roots were observed in infected carrots and witches´ broom in chamomile. Only periwinkles showed virescence and phyllody. On the basis of RFLP analyses of partial 16S rDNA, ribosomal protein rpl22 and tuf (EF-Tu) genes, ACLL phytoplasma was classified in group 16SrI (‘Ca. Phytoplasma asteris’), new subgroup 16SrI-S (rr-rp) tuf-H. All ACLL phytoplasma strains have 16S rRNA interoperon sequence heterogeneity as shown by PCR-RFLP and nucleotide sequence analyses. Phylogenetic analysis using 16S rDNA sequences clustered ACLL phytoplasma strains into a new phylogenetic lineage within group 16SrI.

Keywords


interoperon sequence heterogeneity; genetic polymorphism; sequencing; aster yellows subgroups; PCR-RFLP

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DOI: http://dx.doi.org/10.4454/jpp.v93i2.1185

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