DIVERSITY OF PLUM POX VIRUS ISOLATES IN MONTENEGRO

M. Virscek Marn, I. Mavric Plesko, J. Zindovic, Z. Miladinovic

Abstract


In 2006 and 2007, stone fruit tree samples collected throughout Montenegro were tested for the presence of Plum pox virus (PPV) by DAS-ELISA. The majority (85%) of the plum and peach samples were infected. The occurrence of PPV was confirmed by immunocapture-RT-PCR using various primer pairs and by sequencing. The sequence of a 908 nt fragment corresponding to the NIb(C-ter)/CP(N-ter) region of 43 isolates was determined and compared with other sequences from the NCBI database. PPV sequences from Montenegro belonged to PPV-Rec (35), PPV-D (7) and PPV-M (1). These results show that the three major PPV strains occur in Montenegro and that PPV-Rec is predominant in plums. This is the first extensive characterization of PPV isolates from Montenegro.

Keywords


PPV strains; Sharka; ELISA; diagnosis; sequencing; phylogenetic analysis

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DOI: http://dx.doi.org/10.4454/jpp.fa.2012.008

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