DETECTION OF RHIZOCTONIA CEREALIS IN WHEAT TISSUES BY A REAL-TIME PCR ASSAY

M.S. Hamada, Y.N. Yin, Z.H. Ma

Abstract


Wheat sharp eyespot (WSE) caused by Rhizoctonia cerealis is one of the most important diseases on wheat in China. In this study, a pair of PCR primers was designed based on the sequence of a DNA region amplified by the microsatellite primer M13. The primer pair amplified a 272 bp fragment from all R. cerealis isolates collected from wheat stems at different locations in different years, but not from any other fungal species tested. A real-time PCR assay using this primer pair was developed to detect early and suspected infection of R. cerealis on wheat stems in the field. The real-time PCR assay established here could help verifying the presence of R. cerealis in stem-base diseased wheat plants, thus assisting growers in choosing the proper fungicides.

Keywords


Real-time polymerase chain reaction; Rhizoctonia cerealis; Wheat sharp eyespot

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DOI: http://dx.doi.org/10.4454/jpp.fa.2012.016

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