FIRST REPORT OF TOBACCO VEIN BANDING MOSAIC VIRUS INFECTION OF WILD EGGPLANT IN CHINA

P. Zhou, S.Y. Zhang, N.T. Yu, Y.L. Zhang, J.H. Wang, Z.X. Liu

Abstract


Tobacco vein banding mosaic virus (TVBMV), a member of the genus Potyvirus, mainly infects solanaceous plants, including tobacco (Habera et al., 2000), Datura stramonium (Roggero et al., 2000), and potato (GenBank accession No. DQ917752). In autumn 2013, two plants of wild eggplant (Solanum torvum) displaying vein banding and leaf spot were collected from the province of Guizhou (China). Total RNA was extracted with TRIzol (Invitrogen, USA) and reverse transcribed to first-strand cDNA with primer M14T (5’-GTTTTCCCAGTCACGACTTTTTTTTTTTTTTTTTT-3’) and random primers. A specific product of 1700 bp containing the partial sequence of the NIb (ca. 950 bp) and CP (ca. 750 bp) coding regions was amplified by PCR with degenerate potyvirus primer pairs F:5’-GTITGYGTKGAYGAYTTYAAYAA-3’ and R:5’-GTRTGBCKYTCIGTRTYYTC-3’. Nucleotide sequence analysis confirmed the presence of the suspected TVBMV (GenBank accession No. KJ010807) with 98%, 92% and 91% nucleotide sequence identity with TVBMV isolates YN9.1 (KF444434), YND (EF219408) and JX (JN630471), respectively. To the best of our knowledge, this is the first report of TVBMV on Solanum torvum.

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DOI: http://dx.doi.org/10.4454/JPP.V96I2.009

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