GENETIC CHARACTERIZATION OF THE RICE SHEATH BLIGHT PATHOGEN RHIZOCTONIA SOLANI AG1-IA IN NORTH VIETNAM BY REP-PCR AND SEQUENCE ANALYSIS

M. Matsumoto, H.V. Cuong

Abstract


Representative isolates of Rhizoctonia solani AG1-IA associated with rice sheath blight obtained from North Vietnam were characterized using rep-PCR fingerprinting and ITS-5.8S rDNA sequencing. For rep-PCR assays primers ERIC2 and BOXA1R were used. Sequences of these isolates were aligned with other known R. solani sequences from the GenBank. Rep-PCR analysis revealed that two genotypes (Rep-Vet1 and Rep-Vet2) were present based on the fingerprinting dendrograms having the similarity coefficient score at 75% differentiation. The two clonal populations were scattered and had different geographical localizations in North Vietnam.

Keywords


Rep-PCR;Rhizoctonia solani AG-1 IA;Rice;Sheath blight

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DOI: http://dx.doi.org/10.4454/JPP.V96I2.018

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