RAPID DETECTION OF APPLE STEM GROOVING VIRUS BY REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

L. Zhao, C.-H. Feng, B.-Q. Li, X.-A. Hao, H. Liu, Y.-F. Wu, Q.-C. Wang

Abstract


Apple stem grooving virus (ASGV) is one of the relevant apple viruses worldwide. In this study, a simple, sensitive one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of ASGV, using primers designed in the coat protein gene. The RT-LAMP assay identified 53 infected apple leaf samples while RT-PCR identified only 49 infected samples. To the best of our knowledge, this is the first report on detection of ASGV by RT-LAMP.

Keywords


Apple stem grooving virus;LAMP;detection;diagnosis

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DOI: http://dx.doi.org/10.4454/JPP.V96I2.023

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EDIZIONI ETS, Pisa, Italy