HETEROGENEITY IN FUSARIUM SPECIES AS REVEALED BY INTER-RETROTRANSPOSON AMPLIFIED POLYMORPHISM (IRAP) ANALYSIS

M.I.E. Arabi, M. Jawhar

Abstract


In the present study, 24 Fusarium spp. isolates (8 Fusarium culmorum, 4 F. solani, 9 F. verticillioides and 3 F. equiseti) collected from wheat grown in Syria were analyzed using inter-retrotransposon amplified polymorphism (IRAP), a technique based on the amplification of genomic regions between long terminal repeats. IRAP analysis allowed differentiation between species on the basis of the banding patterns. A total of 844 bands were scored, 421 (49.88%) of which were polymorphic. A neighbour-joining diagram, based on Nei’s genetic distance, showed that isolates formed four phylogenetic groups, but did not fall into clusters or clades correlated with the origin or colour of the isolate, which suggests regional dispersal of these species. The level of genetic variability detected within Fusarium isolates confirmed that IRAP is a reliable, efficient, and effective marker technology for determining genetic relationships within the genus Fusarium.

Keywords


Fusarium spp.; wheat; phylogenetic analysis; IRAP marker; DNA fingerprinting

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DOI: http://dx.doi.org/10.4454/jpp.v92i3.322

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