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LEEK YELLOW STRIPE VIRUS ISOLATES FROM BRAZIL FORM A DISTINCT CLADE BASED ON THE P1 GENE

D. Bampi, T. Mituti, M.A. Pavan, J. Hammond, R. Krause-Sakate

Abstract


The complete genome sequence of a garlic isolate of Leek yellow stripe virus (LYSV) from Brazil (LYSV-MG) was determined and its phylogenetic relationships with other LYSV isolates from other parts of the world were inferred. The LYSV-MG genome consists of 10,341 nucleotides and encodes a polyprotein of 3,221 amino acids. Brazilian LYSV isolates are more closely related to S-type than to N-type isolates but do not have a deletion in the P1 gene. Partial nucleotide sequence analysis of the P1 gene revealed 97-99% identity among Brazilian isolates, and 51-64% identity between Brazilian isolates and isolates from other countries for which sequence information is available in Genbank. The Brazilian isolates formed a monophyletic group closest to the S-type isolates and one isolate from Okinawa (O-type). These data suggest that Brazilian LYSV isolates are derived from an ancestral source of the Okinawa and S-type isolates, prior to the occurrence of the P1 deletion and divergence of the S-type isolates.

Keywords


Allium sativum; Potyvirus; variability; classification

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DOI: http://dx.doi.org/10.4454/JPP.V97I3.021

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