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GENETIC DIVERSITY OF CHILEAN ISOLATES OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 2

A. Zamorano, X. González, N. Quiroga, N. Fiore

Abstract


Grapevine leafroll-associated virus 2 (GLRaV-2) is prevalent in Chile but no information is available on the genetic variability of Chilean isolates. Three genomic regions, i.e. a partial RNA-dependent RNA polymerase (RdRp) gene, the complete coat protein (CP) gene and a partial p19 gene fragment, of eleven Chilean isolates were used for phylogenetic analyses. The genetic identity among Chilean isolates was high (73.7 to 100% for CP-p19 region and 78.7 to 99.8 for RdRp region) and the isolates clustered into four (RG, PN, 93/955 and BD) of the five recognized GLRaV-2 lineages. The RdRp and CP-p19 nucleotide sequences of isolate 6139-LR2-PN showed different phylogenetic relationship, suggesting its recombinant nature. To our knowledge, this is the first genetic analysis of Chilean isolates of GLRaV-2.

Keywords


RT-PCR; Recombinant GLRaV-2; RdRp gene; CP gene; p19 protein; sequencing

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DOI: http://dx.doi.org/10.4454/JPP.V98I1.042

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