FIRST REPORT OF POTATO YELLOWING VIRUS INFECTING PEPPER IN ECUADOR

Y. Sivaprasad, P. Garrido, K. Mendez, A. Garrido, L. Ramos

Abstract


Pepper (Capsicum annum L.) is a solanaceous species originating from South and Central America. Potato yel- lowing virus (PYV), an unclassified putative member of the genus Ilarvirus, was first found in potato in Peru (Fuentes et al., 1993), later in wild potato (Solanum fernandezianum) in Chile (Valkonen et al., 1992) and more recently in a native potato (Solanum phureja) in Ecuador (Silvestre et al., 2011). In December 2014, foliar mosaic and necrotic spotting of leaves and stems accompanied by bud and fruit necrosis were observed in pepper fields of Puembo, in the Pichincha prov- ince of Ecuador. Based on the symptomatology, the occur- rence of PYV was suspected and confirmed in symptomatic leaves by DAS-ELISA with a specific antiserum provided by G. Muller, International Potato Center, Lima, Peru, and by RT-PCR using universal primers for members of the family Bromoviridae (Untiveros et al., 2010). The 300 bp amplicon obtained from a symptomatic pepper was purified, custom- sequenced (Macrogen, South Korea) and the nucleotide se- quence was deposited in GenBank (KP772263). Sequence analysis (BioEdit v. 7.05) showed 93.1-97% and 78-90% iden- tity with other PYV isolates at the nucleotide and amino acid levels, respectively. A phylogenetic tree constructed at the nucleotide level using MEGA version 4.1 showed that the Ecuadorian PYV isolate from capsicum was closely related to isolate Loja 2 from S. phureja in Ecuador (HQ141056). Solanum isolates Canete (HQ141057), Russia (KM244740), Loja 1 (HQ141053) and Azuay (HQ141054) formed a sepa- rate cluster. To the best of our knowledge, this is the first report of PYV on Capsicum in Ecuador.

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DOI: http://dx.doi.org/10.4454/JPP.V97I4SUP.023

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