DAC-ELISA AND RT-PCR BASED CONFIRMATION OF SYSTEMIC AND LATENT INFECTION BY TOBACCO STREAK VIRUS IN COTTON AND PARTHENIUM

S. Rageshwari, P. Renukadevi, V.G. Malathi, P. Amalabalu, S. Nakkeeran

Abstract


Tobacco streak virus (TSV), the causal agent of cotton necrosis, is an emerging disease of national significance in India. Owing to its significance, cotton plants cultivated in Tamil Nadu, India were surveyed for cotton necrosis during 2015-2016. The samples collected from different cotton growing tracts of Tamil Nadu were observed for the presence of symptoms associated with TSV infection in cotton (Gossypium hirsutum and Gossypium barbadense). Serological assay by Direct Antigen Coating-Enzyme Linked Immuno Sorbent Assay (DAC-ELISA) revealed the presence of TSV in leaf, square, stem, root, petiole and pollen grains of both asymptomatic and symptomatic samples of variety CO 14 of cotton. Similarly, the presence of TSV in different plant parts including root, stem, leaf, pollen grains and squares in both asymptomatic and diseased plants was confirmed through Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). All the samples collected from both asymptomatic and symptomatic samples yielded the expected PCR product of 929 bp including to the coat protein gene of TSV and thus confirmed the systemic nature of the virus. Since parthenium (Parthenium hysterophorus) serves as a reservoir of TSV, attempts were also made to confirm the systemic nature of TSV in asymptomatic parthenium plants. DAC- ELISA and RT-PCR based detection tools confirmed the presence of TSV in the asymptomatic leaf, stem and pollen grains.

Keywords


Tobacco streak virus; growth and yield parameters; systemic infection in cotton and parthenium

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DOI: http://dx.doi.org/10.4454/jpp.v99i2.3876

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