IDENTIFICATION OF COTTON LEAF CURL KOKHRAN VIRUS AND MULTIPLE SATELLITE MOLECULES INFECTING JASMINUM SAMBAC IN PAKISTAN

A. Akram, G. Rasool, A. Rehman, S. Mansoor, R.W. Briddon, M. Saeed

Abstract


Jasminum sambac is grown as an ornamental across Southern Asia. Amongst a group of 15 J. sambac plants growing next to cotton fields in Faisalabad in 2016, four were exhibiting mild leaf curling, yellowing and vein-thickening, typical of begomovirus infection. DNA was extracted from the leaves of two symptomatic and one non-symptomatic plant using the CTAB method. DNA fragments of ca. 2.8 kb were PCR amplified and cloned from the symptomatic (plants p1 and p2) and non-symptomatic (plant p3) plants with begomovirusspecific primers BF/BR (Mubin et al., 2011). The sequences of three clones (GenBank accession Nos. KY797661-KY797663 from p1 to p3, respectively) showed 99% nucleotide sequence identity with cotton leaf curl Kokhran virus (CLCuKoV; AJ496286). Betasatellites and alphasatellites were amplified using primers beta01/beta02 and DNA101/DNA102 (Mubin et al., 2011). The sequences of two betasatellites [KY797668 (p2) and KY797669 (p3)] and a partial sequence (p1) showed 99% sequence identity with cotton leaf curl Multan betasatellite (CLCuMuB; FN432359). Three alphasatellite clones (KY797664-KY797666, from p1, p1 and p3, respectively) had 99% sequence identity with guar leaf curl alphasatellite (HE599396) and one (KY797667; p3) had 98% sequence identity with okra leaf curl alphasatellite (AJ512954). Marwal et al. (2013) previously showed J. sambac in India to be infected with a begomovirus based on partial sequences. This is the first report of J. sambac being a host for CLCuKoV, CLCuMuB and alphasatellites which are associated with cotton leaf curl disease in South Asia. This shows J. sambac is an alternative, sometimes asymptomatic host for economically important, crop-infecting begomoviruses on the subcontinent.

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DOI: http://dx.doi.org/10.4454/jpp.v99i3.3967

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