VIRUSES AND VIROIDS OF STONE FRUITS IN EGYPT

I. El Maghraby, S. Matić, H. Fahmy, A. Myrta

Abstract


Field surveys were carried out to assess the sanitary status of stone fruits in Egypt. A total of 716 samples was tested by ELISA for Prunus necrotic ring spot virus (PNRSV), Plum pox virus (PPV), Prune dwarf virus (PDV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). The viruses most frequently detected were PNRSV (57%) and PPV (41%); PDV and ACLSV were found in 3% of samples and ApMV was not detected. Plum bark necrosis stem pitting-associated virus (PBNSPaV) and Apricot latent virus (ApLV) were detected for the first time in Egypt by using RTPCR. Of 693 samples tested by tissue-imprint hybridization (TIH), 101 (15%) were found to be infected by either Peach latent mosaic viroid (PLMVd) or Hop stunt viroid (HSVd).

Keywords


Prunus; ELISA; RT-PCR; tissue-imprint hybridization; sanitary status

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DOI: http://dx.doi.org/10.4454/jpp.v89i3.777

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