RT-PCR DETECTION AND PROTEIN-PROTEIN INTERACTION OF VIRAL COMPONENTS OF PINEAPPLE MEALYBUG WILT-ASSOCIATED VIRUS 2 IN CUBA

E.G. Borroto-Fernández, J.A. Torres-Acosta, M. Laimer

Abstract


During a search for the causal agent of symptoms of mealybug wilt in Cuban pineapple plants, Pineapple mealybug wilt-associated virus 2 (PMWaV-2) was detected. A reverse transcription polymerase chain reaction (RT-PCR) test was developed to amplify seven ORFs using primers designed on the RNA sequence of a Hawaiian isolate of PMWaV-2. Cuban and the Hawaiian PMWaV-2 isolates shared high sequence homology. Yeast two-hybrid assays showed the homodimeric nature of the 20 kDa protein (p20) and the 22 kDa protein (p22), but not of the 34 kDa coat protein (CP). This is the first report of protein-protein interactions between isolated PMWaV-2 proteins.

Keywords


Ampelovirus; electron microscopy; Pineapple mealybug wilt-associated virus-2; yeast two-hybrid system

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DOI: http://dx.doi.org/10.4454/jpp.v89i3.779

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