POLYPEPTIDE PATTERNS OF SYRIAN ISOLATES OF PYRENOPHORA GRAMINEA

M.I.E. Arabi, M. Jawhar, N. MirAli

Abstract


Pyrenophora graminea, the causal agent of leaf stripe of barley, is an economically important disease with a worldwide distribution. This fungus is highly variable thus information regarding polypeptide patterns is essential in studying population genetic structure. To establish the number and distribution of polypeptide patterns of Syrian populations of P. graminea, 61 isolates from diverse regions were analyzed using 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Twelve unique patterns were identified. The most common pattern was p11, which comprised 44% of the isolates examined. In general, northern Syrian isolates exhibited a broader spectrum of polypeptide patterns than those from other regions of the country. Identical numbers of polymorphic bands were observed in all assays (for each isolate/polypeptide pattern). Our results confirm the potential of polypeptide patterns for the molecular characterization of P. graminea, and their usefulness in future selection of isolates to develop durable leaf stripe resistance.

Keywords


Pyrenophora graminea; Barley; SDSPAGE; polypeptide patterns

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DOI: http://dx.doi.org/10.4454/jpp.v88i2.858

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