PRODUCTION OF DIAGNOSTIC ANTIBODIES TO PLANT VIRUSES, UTILIZING DENATURED COAT PROTEINS AND BOOSTER IMMUNIZATION WITH PARTIALLY PURIFIED VIRUS

A. Gera, J. Cohen, A. Kritzman, E. Beckelman, M. Bar-Joseph

Abstract


Groups of rabbits were immunized intramuscularly with either partially purified or denatured Iris Yellow Spot Virus (IYSV) nucleocapsid protein (NC) excised from SDS-polyacrylamide gels. Four injections were given at weekly intervals. Two weeks later, booster injections were given either with partially purified preparations of native virions or with denatured NC. Twelve days after the booster, rabbits were bled and the binding capacity of antisera was examined by decoration tests, Western blotting and ELISA. Diagnostic antibodies which could specifically bind to virus particles were obtained. Higher ELISA values were obtained when the booster injection contained partially purified IYSV, rather than denatured NC. Similar results were obtained with Potato Virus Y.

Keywords


Iris Yellow Spot; Potato Virus Y; diagnostic antibodies; coat protein; decoration; ELISA; immunoblot

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DOI: http://dx.doi.org/10.4454/jpp.v81i3.1075

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