AN ARGENTINEAN ISOLATE OF LEEK YELLOW STRIPE VIRUS FROM LEEK CAN BE TRANSMITTED TO GARLIC

P. Lunello, D.A. Ducasse, M. Helguera, S.F. Nome, V.C. Conci

Abstract


Leek yellow stripe virus (LYSV) causes mosaic in garlic and leek, together with other potyviruses, carlaviruses and allexiviruses. An isolate of LYSV from leek plants presenting typical yellow stripes, near Córdoba, Argentina, was mechanically transmitted and caused chlorotic local lesions in Chenopodium amaranticolor and C. quinoa. Only two out of 40 onion plants mechanically inoculated were infected. The isolate was easily transmitted to garlic mechanically (14 out of 20 plants) and by aphids (2 out of 10 plants), especially to young plants. Myzus persicae, Rhopalosiphum maidis, R. padi, Schizaphis graminum, Aphis gossypii, A. nerii, Uroleucon sonchi and Hyperomyzus carduellinus were able to transmit the virus. Abundant inclusion bodies were observed in thin sections. The modal length of the virion was 825 nm, and the capsid protein was 34 kDa in size, as determined by SDS-PAGE. LYSV from leek and Onion yellow dwarf virus were compared using several antisera, including one prepared against our isolate and their serological relatedness confirmed. The 3´-nontranslated region (3´-NTR) of our isolate (accession no. AY007693) showed 94% identity with that of LYSV (accession no. X89711) and the deduced amino acid sequence for the coat protein had 97% homology.

Keywords


Allium ampeloprasum var. porrum; antiserum production; aphid transmission; nucleotide sequence

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DOI: http://dx.doi.org/10.4454/jpp.v84i1.1082

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