SEROLOGICAL CHARACTERISATION OF MEDITERRANEAN PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES

A. Myrta, B. Di Terlizzi, D. Boscia, E. Choueiri, M. Gatt, I. Gavriel, K. Çaglayan, C. Varveri, H. Zeramdini, F. Aparicio, V. Pallás, V. Savino

Abstract


Prunus necrotic ringspot virus (PNRSV), a worldwide pathogen of stone fruits, has many isolates with different biological, serological, and molecular properties. Monoclonal antibodies (MAbs) to a peach isolate of PNRSV were used to investigate the serological variability of PNRSV isolates by DASI-ELISA. Isolates from different stone fruit species and different Mediterranean countries were first identified with polyclonal antisera (PAbs), then 81 isolates were selected from Albania (3), Cyprus (10), Greece (5), Italy (31), Lebanon (6), Malta (5), Tunisia (7), Turkey (7) and Spain (1) and characterised against 10 single MAbs. Six U.S. isolates were included as controls. The virus showed high serological variability as shown by the identification of 34 serogroups, many of which were host-specific (64%) and country-specific (67%).

Keywords


PNRSV; MAbs; serological characterisation; serogroups; DASI-ELISA

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DOI: http://dx.doi.org/10.4454/jpp.v83i1.1111

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