COMPARISON OF CHERRY GREEN RING MOTTLE VIRUS STRAINS USING RT-PCR AND COAT PROTEIN SEQUENCE PHYLOGENY

Y.P. Zhang, B.C. Kirkpatrick, B. Di Terlizzi, J.K. Uyemoto

Abstract


Assays for Cherry green ring mottle virus (CGRMV) were developed and used to compare different sources from North American and Mediterranean countries by differential reverse transcription-polymerase chain reaction (RT-PCR) amplification and phylogenetic analysis of coat protein sequences. DsRNAs of approximately 8.5 kb in size were purified from all diseased samples. Universal and specific primer pairs derived from sequences of two Californian strains of CGRMV were used in RT-PCR assays. Parsimony analyses using coat protein nucleotide sequences separated the 12 CGRMV sources into three major phylogenetic clades.

Keywords


Cherry; green ring mottle viruses; Foveovirus; RT-PCR; molecular cloning.

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DOI: http://dx.doi.org/10.4454/jpp.v82i1.1144

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