PROPERTIES OF TWO MONOCLONAL ANTIBODIES SPECIFIC TO THE CHERRY STRAIN OF PLUM POX VIRUS

A. Myrta, O. Potere, A. Crescenzi, M. Nuzzaci, D. Boscia

Abstract


Two monoclonal antibodies (MAbAC and MAb- TUV) raised against the sweet cherry isolate of Plum pox virus (PPV-SwC) were tested against homologous and heterologous PPV strains from different geographical origins. Both MAbs proved to be PPV-C (Cherry) strain-specific. They reacted in DASI-ELISA with PPVSwC and the sour cherry isolate of PPV (PPV-SoC) from Nicotiana benthamiana, but not with any of additional 44 PPV isolates tested belonging to the Dideron, Marcus and El Amar strains. The strain specificity of both MAbs was also assessed by Western blot and immuno electron microscopy. As indicated by competitive ELISA, the two MAbs are elicited by different epitopes.

Keywords


cherry; Plum pox virus; PPV-C; strainspecific MAbs; ELISA; Western blot.

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DOI: http://dx.doi.org/10.4454/jpp.v82i2.1148

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