IMMUNODETECTION OF THE 20 kDa PROTEIN ENCODED BY ORF 2 OF GRAPEVINE VIRUS B

P. Saldarelli, A. Minafra

Abstract


A polyclonal antiserum was raised to the 20 kDa product encoded by ORF2 of Grapevine virus B (GVB) using a recombinant protein. A rabbit was immunized with the polypeptide expressed in Escherichia coli fused with gluthatione-S-transferase. The antiserum successfully detected the 20 kDa protein in Nicotiana occidentalis plants in the late stages of infection, an accumulation pattern that differs substantially from that of GVB movement protein.

Keywords


words: grapevine; polyclonal antiserum; virus; GVB; recombinant protein; immunodetection.

Full Text:

PDF


DOI: http://dx.doi.org/10.4454/jpp.v82i2.1156

Refbacks

  • There are currently no refbacks.


EDIZIONI ETS, Pisa, Italy