BACTERIAL LEAF BLIGHT OF CHRIST’S THORN IN IRAN: A NEW DISEASE CAUSED BY SPHINGOMONAS sp.

M. Deldavleh, K. Bahmani, B. Harighi

Abstract


A bacterial leaf blight of Christ’s thorn plants (Paliurus spina-christi) observed in the Kurdistan province of Iran is described for the first time. Main symptoms of the disease are foliar water-soaked, irregular spots with yellow halo, 5-8 mm in diameter, which eventually turn brown. Due to it is relatively recent detection in Iran, very little is known about the pathogen. Yellow, non-mucoid colonies consistently isolated from leaf lesions were produced by a Gram-negative, catalase positive and oxidase negative bacterium, 15 isolates of which showed more than 90% similarity. Comparative 16S rDNA gene sequence analysis positioned this bacterium in the genus Sphingomonas with the highest sequence similarity (97%) to Sphingomonas melonis. Chemotaxonomically, fatty acid methyl ester analysis revealed that the bacterial isolates contained C16:0 (9%), C18:1 ώ 9 (51%) as predominant fatty acids and C14:0 2OH (25%) as a major 2-hydroxy fatty acid, confirming their belonging to the genus Sphingomonas.

Keywords


16S rDNA sequence analysis;FAME analysis;Paliurus spina-christi;Sphingomonas

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DOI: http://dx.doi.org/10.4454/JPP.V95I1.018

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EDIZIONI ETS, Pisa, Italy