FIRST REPORT OF TWO DISTINCT STRAINS OF PEPINO MOSAIC VIRUS INFECTING TOMATOES IN GREENHOUSES IN LITHUANIA

D. Šneideris, M. Žižitė, I. Zitikaitė, L. Urbanavičienė, J. Staniulis

Abstract


During 2010 and 2011, one hundred thirty one symptomatic samples of tomato leaves and fruits were collected from commercial greenhouses in Lithuania and analyzed for the presence of Pepino mosaic virus (PepMV). Two isolates of this virus (NV and KK) were found in two different greenhouses on a tomato plant exhibiting mild yellow leaf spotting and on tomato fruits with marbling symptoms, respectively. The presence of the virus was confirmed by RT-PCR and DAS-ELISA, using commercial antibodies (DSMZ, Germany). Filamentous virus particles were observed by electron microscopy. The coat protein (CP) gene of the two viral isolates was amplified using a two-step RT-PCR with the PepTGB-F/PepUTR-R primer pair (Mumford and Metcalfe, 2001). A specific 845 bp PCR product was obtained, purified and sequenced. The nucleotide sequence of the CP gene of PepMV-NV and -KK was submitted to GenBank under the accession numbers JQ979169 and JQ979170, respectively. Nucleotide sequence analysis showed that the two Lithuanian PepMV isolates differ from each other and belong to two distinct PepMV genotypes. PepMV-NV belongs to the EU genotype while PepMV-KK was assigned to the CH2 genotype. These results are consistent with an expansion of the distribution of PepMV in many European countries, including Poland (Pospieszny et al., 2008). They also show that both PepMV EU and CH2 genotypes are becoming common in Europe.

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DOI: http://dx.doi.org/10.4454/JPP.V95I1.024

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