A SIMPLE METHOD FOR DETECTION OF POLYMYXA BETAE AND BEET NECROTIC YELLOW VEIN VIRUS IN SOIL

A. Ghorbani, K. Izadpanah, F. Manzari, V. Roumi

Abstract


Extraction of high quality DNA and RNA from soil is a most challenging step to identify soil organisms due to the presence of a variety of inhibitors and microbial nucleases. In this study, we have developed a simple extraction method for simultaneous purification of DNA and RNA from soil. The isolated nucleic acids were of high quality allowing for sensitive detection of the oomycete Polymyxa betae and Beet necrotic yellow vein virus (BNYVV) by nested-PCR. The vector and BNYVV were detected in all infested soils but only the vector was detected in some apparently non-infested soils or fallow fields. The method may help in the management of sugar beet rhizomania.

Keywords


disease management;nucleic acid extraction;oomycetes;soil-borne viruses

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DOI: http://dx.doi.org/10.4454/JPP.V95I3.012

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EDIZIONI ETS, Pisa, Italy