INCIDENCE OF MAJOR PEACH VIRUSES AND VIROIDS IN CHINA

Y. Yu, Z. Zhao, L. Qin, Y. Zhou, H. Fan, Z. Zhang, Z. Wu, S. Li

Abstract


Field surveys were carried out in the main peach-growing areas of China to assess the virus or viroid disease status of peach trees. A total of 505 leaf samples were tested by ELISA or RT-PCR for the presence of nine major peach viruses. The viruses most frequently detected were Apple chlorotic leaf spot virus (ACLSV) (71.8%), followed by Prunus necrotic ringspot virus (PNRSV) (20.2%), Cherry green ring mottle virus (CGRMV) (8.1%), and Apricot pseudo-chlorotic leaf spot virus (APCLSV) (1.6%). The overall average of virus infection level in peach trees was 24.6%. Apple mosaic virus (ApMV), Plum pox virus (PPV), Prune dwarf virus (PDV), Cherry virus A (CVA) and Cherry leaf roll virus (CLRV) were not detected. Of another 583 leaf samples tested for the presence of the known major peach viroids, by dot-blot hybridization, 444 (76.2%) were infected. Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) were detected in 443 and 18 samples, respectively. This is the first large-scale study on major viruses and viroids that infect peach trees in China.

Keywords


Peach;ELISA;RT-PCR;dot-blot hybridization;virus detection;viroids

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DOI: http://dx.doi.org/10.4454/JPP.V95I3.045

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