ONION YELLOW DWARF VIRUS ON LEEK, ONION, SHALLOT AND WELSH ONION IN IRAN

Z. Naderi Saffar, S. Torabi, M. Naghavi, A.R. Golnaraghi, E. Aryakia

Abstract


During summer of 2011-2012, bulb samples from allia- ceous vegetables, i.e. garlic (Allium sativum), leek (Allium porrum), onion (Allium cepa), shallot (Allium hirtifolium) and Welsh onion (Allium fistulosum) were collected from Az- arbaijan-e-sharghi, Hamedan, Kerman, Khuzestan, Lorestan, Mazandaran, Markazi and Zanjan provinces of Iran to de- termine the presence of Onion yellow dwarf virus (OYDV), genus Potyvirus. Collected bulbs were planted in plastic pots and the resulting plantlets were tested individually at the two-leaf stage by indirect ELISA using the “Poty group test” kit from Bioreba (Switzerland). ELISA-positive samples were subsequently tested by RT-PCR using previously de- scribed specific primers to amplify a region in the 3’ end of the OYDV genome (Van der Vlugt et al., 1999). RT-PCR resulted in the amplification of an expected fragment of ca. 0.5 kb in size for 30 out of 35 ELISA-positive seedlings from garlic, leek, onion, shallot and Welsh onion bulbs, confirm- ing the presence of OYDV. Symptoms of mosaic and yellow streak striping, curling and distortion of flower stems were associated with potyvirus infections. OYDV was previously reported on garlic in Iran (Baghalian et al., 2010) but this is the first report of its natural occurrence on leek, onion, shal- lot and Welsh onion in the mid-Eurasia of Iran.

Full Text:

PDF


DOI: http://dx.doi.org/10.4454/JPP.V95I4.013

Refbacks

  • There are currently no refbacks.


EDIZIONI ETS, Pisa, Italy