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DETECTION OF GRAPEVINE PINOT GRIS VIRUS IN CERTIFIED GRAPEVINE STOCKS IN MORAVIA, CZECH REPUBLIC

A. Eichmeier, E. Peňázová, R. Pavelková, Z. Mynarzová, P. Saldarelli

Abstract


Twenty one grapevine mother plants used by nurseries for propagation in South Moravia, Czech Republic, were tested for the presence of Grapevine Pinot gris virus (GPGV) and other viruses by simplex and multiplex RT-PCR. GPGV was found in all vines tested and Grapevine virus A, Grapevine fanleaf virus and Grapevine fleck virus were detected only in some of them. Part of the movement and coat protein coding regions of 21 GPGV isolates was sequenced. Phylogenetic analysis revealed that south Moravian GPGV isolates grouped with isolates from other regions and countries. This study provides the first comprehensive survey of the GPGV occurrence in South Moravia.

Keywords


Grapevine Pinot gris virus; sequencing; RT-PCR

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DOI: http://dx.doi.org/10.4454/JPP.V98I1.043

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