IDENTIFICATION OF A NATURALLY OCCURRING BEAN COMMON MOSAIC VIRUS RECOMBINANT ISOLATE INFECTING AZUKI BEAN

Abstract

The near complete nucleotide sequence of Bean common mosaic virus (BCMV) infecting azuki bean from China was determined to be 10047 nucleotides in length excluding the 3′-terminal poly (A) sequence. The isolate was designated as BCMV-Az and caused stunting, mosaic and crumpling on leaves of azuki plants. It contains a large open reading frame (ORF) flanked by a 131 nt 5′-untranslated region (UTR) and a 253 nt 3′-UTR and the putative polyprotein encoded by this large ORF is comprised of 3220 amino acid residues. Phylogenetic analysis showed that BCMV-Az clustered with strains isolated from different hosts and geographic origins, suggesting that it is a novel strain. Recombination analyses indicated that it was a recombinant originating from isolates R (AJ312437) and US10 (KF919299).