Characterisation of polyclonal antibodies raised against two isolates of cucumber mosaic virus

H. Berniak, T. Malinowski, M. Kaminska

Abstract


Antisera were raised in rabbits against two isolates of Cucumber mosaic virus (CMV), representing groups I (DTL) and II (ToRS). The reactions of these polyclonal antibodies (PAbs) with several CMV isolates were checked, using ELISA, Western blotting and IC-RTPCR. One of the PAbs against isolate M (group I) enabled detection of most of the CMV isolates tested but failed to recognize isolate P26, originating from lily. A PAb against isolate Wic (group II) reacted also with all isolates tested, showing up to 100-fold improvement in detection sensitivity compared with two other CMVspecific PAbs. The coat protein (CP) of CMV isolates belonging to both groups was detected by Western blotting using M and Wic antisera. The suitability of a mixture of PAbs for immunocapturing CMV and for the sensitive detection of all viral isolates tested by IC-RTPCR was also shown.

Keywords


CMV; serology; polyclonal antisera; ELISA; IC-RT-PCR; Western blotting

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DOI: http://dx.doi.org/10.4454/jpp.v92i1.35

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