CHARACTERIZATION OF A SUBGROUP II ISOLATE OF CUCUMBER MOSAIC VIRUS FROM BITTER GOURD IN CHINA

L. Zhu, Z.P. Xu, C.Y. Fei, D.H. Xi

Abstract


Diseased bitter gourd plants with severe leaf rolling symptoms were observed in Sichuan province (southwest China). Leaf dips-prepared from symptomatic leaves contained particles ca. 30 nm in diameter. The virus recovered from all samples was identified as Cucumber mosaic virus (CMV) by Western blotting, RT-PCR and sequencing of amplicons. All obtained sequences were identical, and the virus isolate was designated SC-J1. The complete nucleotide sequence of the CP (GenBank accession No. KT932936) of the isolate shared 99% identity to the corresponding regions of strain Hnt (CMV II). Phylogenetic analysis based on the nucleic acid sequence of the CP shows that SC-J1 belongs to CMV subgroup II. To our knowledge, this is the first report of CMV II infecting bitter gourd in China.

Keywords


bitter gourd; electron microscopy; Western blot; RT-PCR

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DOI: http://dx.doi.org/10.4454/jpp.v99i2.3870

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