TRACKING THREE ILARVIRUSES IN STONE FRUIT TREES THROUGHOUT THE YEAR BY ELISA AND TISSUE-PRINTING HYBRIDIZATION

S. Matic, J.A. Sánchez-Navarro, B. Mandic, A. Myrta, V. Pallás

Abstract


The presence of Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV) and Apple mosaic virus (ApMV) was monitored throughout 2005 in stone fruit trees by ELISA and tissue printing hybridization. The highest detection rate for all three viruses was obtained in winter with both techniques. Detection was reliable also in spring and early summer, except for Ap- MV. However, neither technique was able to identify successfully all infected samples in one season. Overall, ELISA was more successful for PNRSV and PDV detection and tissue-printing hybridization for ApMV.

Keywords


PNRSV; PDV; ApMV; diagnosis; ELISA; tissue-printing hybridization

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DOI: http://dx.doi.org/10.4454/jpp.v90i1.605

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