A NOVEL INOCULATION METHOD FOR EVALUATION OF GREY LEAF SPOT RESISTANCE IN ITALIAN RYEGRASS

W. Takahashi, Y. Miura, T. Sasaki

Abstract


We developed a novel inoculation method, the ‘filterpaper method’, for the assay of grey leaf spot (GLS) in Italian ryegrass (Lolium multiflorum Lam.). In this method, a conidial suspension of Pyricularia oryzae, the causal agent of GLS, is dropped onto a filter paper, which is placed, inoculated-side down, on the abaxial surface of a detached leaf on an agar plate. The filter-paper method was more repeatable than the spot inoculation method developed for rice, and the disease scores obtained were correlated (r = 0.6, P < 0.01) with those obtained under glasshouse conditions by the standard inoculation method. We optimized this novel method and demonstrated its usefulness for the selection of GLS resistance in Italian ryegrass.

Keywords


Artificial inoculation; ryegrass blast; detached leaf; Magnaporthe oryzae

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DOI: http://dx.doi.org/10.4454/jpp.v91i1.638

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