MOLECULAR CHARACTERIZATION OF THREE SOIL-BORNE SUGAR BEET-INFECTING VIRUSES BASED ON THE COAT PROTEIN GENE

N. Borodynko, N. Rymelska, B. Hasiów-Jaroszewska , H. Pospieszny

Abstract


Sugar beet (Beta vulgaris L. ssp. vulgaris) hosts the soil-borne viruses Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus (BSBV), Beet soil-borne mosaic virus (BSBMV) and Beet virus Q (BVQ). Only a few isolates of these viruses have been characterized at the coat protein (CP) gene level. In this work, CP gene sequences were determined of three isolates each of BNYVV, BSBV and BVQ from different parts of Poland (Wielkopolska, Mazowsze and Dolny Slask). Phylogenetic analysis showed that Polish BNYVV isolates belong to the A and B type groups. The CP gene of the three BSBV isolates had 98-100% identity with comparable sequences available in GenBank. CP genes of the three BVQ isolates were almost identical to one another (98- 99%) and contained three additional nucleotides, resulting in a further amino acid residue (arginine) at position 86, as reported by Lennefors et al. (2005).

Keywords


Sugar beet; Beet necrotic yellow vein virus; Beet soil-borne virus; Beet virus Q; sequencing; coat protein; phylogenetic analysis

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DOI: http://dx.doi.org/10.4454/jpp.v91i1.641

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