SIMULTANEOUS DETECTION OF ONION YELLOW DWARF VIRUS AND SHALLOT LATENT VIRUS IN INFECTED LEAVES AND CLOVES OF GARLIC BY DUPLEX RT-PCR

S. Majumder, V.K. Baranwal, S. Joshi

Abstract


A duplex RT-PCR was standardized for the simultaneous detection of Onion yellow dwarf virus (OYDV) and Garlic latent virus (GarLV), a synonym for the recognized species Shallot latent virus (SLV) in garlic, which allowed the successful identification of both viruses in cloves and leaves. The titre of OYDV was higher both in leaves and bulbs compared with SLV. In the leaves, OYDV was detected up to an RNA dilution of 10-4 while SLV could be detected up to an RNA dilution of 10-3. Duplex RT-PCR detected both viruses in ten commercial garlic cultivars. The procedure is costeffective and sensitive and will be highly useful in quarantine and certification programmes.

Keywords


Allium sativum; OYDV; SLV; Duplex RTPCR; diagnosis

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DOI: http://dx.doi.org/10.4454/jpp.v90i2.676

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EDIZIONI ETS, Pisa, Italy