CHARACTERIZATION OF ITALIAN POPULATIONS OF XANTHOMONAS CAMPESTRIS pv. CAMPESTRIS USING PRIMERS BASED ON DNA REPETITIVE SEQUENCES

M. Zaccardelli, F. Campanile, C. Moretti, R. Buonaurio

Abstract


One-hundred and forty-one strains of Xanthomonas campestris pv. campestris (Xcc), the causal agent of vascular blackening of crucifers were isolated from different crucifer plants in central and southern Italian regions and characterized for DNA polymorphism by PCR, using primers based on repetitive sequences. By M13-PCR, all strains, except for two, were distributed in three main clusters: a, with similarity index (SI) 0.78, containing 70 strains grouped in 19 haplotypes; b (SI = 0.80) containing 54 strains in 17 haplotypes; g (SI = 0.75) containing 15 strains in 6 haplotypes. By BOX-PCR, all strains were distributed in three main clusters: a (SI = 0.58) containing 102 strains distributed in 9 haplotypes; b (SI = 0.45), including 24 strains distributed in 9 haplotypes; g (SI = 0.37), including 15 strains distributed in 3 haplotypes. A very large variability was observed in these Italian populations of Xcc especially using M13-PCR. No obvious relationships were observed between the strains comprised in each cluster, sub-cluster or haplotype and their geographic origin or host plant.

Keywords


phytobacteria; crucifers; PCR; M13; BOX

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DOI: http://dx.doi.org/10.4454/jpp.v90i2.677

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EDIZIONI ETS, Pisa, Italy