VIRUSES AND VIROIDS OF STONE FRUITS IN ALGERIA

N. Rouag, A. Guechi, S. Matic, A. Myrta

Abstract


Field surveys were carried out in the major stone fruit growing areas of eastern Algeria to assess the sanitary status of stone fruits. A total of 454 samples from peach, apricot, almond, sweet and sour cherry, plum and myrobalan were tested by ELISA or RT-PCR for the presence of Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Apple mosaic virus (ApMV), Apple chlorotic leaf spot virus (ACLSV) Apricot latent virus (ApLV), Cherry virus A (CVA), Cherry green ring mottle virus (CGRMV), and Plum bark necrosis stem pitting-associated virus (PBNSPaV). The overall average infection level was 10.4%. The most frequent virus was PNRSV (56.8%), followed by PDV (27.2%) and ApMV (22.7%). The most infected species was cherry (21.9%) and the less almond (4.4%). ACLSV, ApLV, CVA, CGRMV and PBNSPaV were not detected. Of 531 samples tested for the presence of viroids by tissueprint hybridization, 28 (5.2%) were infected. Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) were detected in 15 and 13 samples, respectively. This is the first large-scale study on viruses and viroids of stone fruits in Algeria and reports for the first time the presence of HSVd in the country.

Keywords


Prunus; ELISA; RT-PCR; tissue-print hybridization; incidence; sanitary status; viroids

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DOI: http://dx.doi.org/10.4454/jpp.v90i2.680

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