MOLECULAR CHARACTERIZATION OF BEGOMOVIRUSES INFECTING LUDWIGIA HYSSOPIFOLIA

J.F. Huang, T. Jiang, X.P. Zhou

Abstract


Two Ludwigia hyssopifolia samples G37 and G38 showing yellow vein symptoms were collected from Nanning, Guangxi province of China. Viruses were detected in each sample by polymerase chain reaction (PCR) using universal primers for the genus Begomovirus. The complete DNA-A sequences of isolates G37 and G38 obtained from each source plant comprised 2758 and 2745 nucleotides, respectively. Both DNA-A molecules have the typical genome organization of begomoviruses and share 63.7% sequence identity. Further sequence comparisons showed that DNA-A sequences of G37 and G38 most resembled those of Ageratum yellow vein Taiwan virus (AF307861, 73%) and Alternanthera yellow vein virus (AM050736, 93.6%), respectively. A satellite DNAß molecule was found to be associated with G37 by using abutting primers beta01 and beta02 specific for DNAß. G37 DNAß consists of 1347 nucleotides and has relatively low nucleotide sequence identity (32.4% to 50.2%) with other previously characterized DNAß molecules. The molecular data suggest that G37 is a distinct begomovirus, for which the name Ludwigia yellow vein virus (LuYVV) is proposed, while G38 is an isolate of Alternanthera yellow vein virus.

Keywords


Begomovirus; DNA-A; DNAβ; Ludwigia hyssopifolia

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DOI: http://dx.doi.org/10.4454/jpp.v88i1.834

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