BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF TUNISIAN ISOLATES OF POTATO LEAFROLL VIRUS

F. Djilani Khouadja, J. Rouzé-Jouan, S. Guyader, M. Marrakchi, H. Fakhfakh

Abstract


Nine Tunisian isolates of Potato leafroll virus (PLRV) were collected from potato plants (cv Spunta) with similar secondary leafroll symptoms. These PLRV isolates were readily transmitted by Myzus persicae (Sulzer) from potato plants to Physalis floridana test plants inducing symptoms of differing severities. In order to investigate the genetic variability between the PLRV isolates, the ORF0 regions of the Tunisian PLRV isolates were sequenced and compared with that of a French isolate (Fr5) that induces mild symptoms and 24 ORF0 PLRV sequences available in Genbank. The Tunisian isolates could be classified in two of the three groupings of the phylogenetic tree obtained. A tentative correlation between symptom expression in P. floridana and specific changes in the ORF0 sequences is discussed.

Keywords


ORF0 sequence; Myzus persicae; Physalis floridana; potato; PLRV symptoms

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DOI: http://dx.doi.org/10.4454/jpp.v87i2.902

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