CERATO-PLATANIN, AN EARLY-PRODUCED PROTEIN BY CERATOCYSTIS FIMBRIATA F.SP. PLATANI, ELICITS PHYTOALEXIN SYNTHESIS IN HOST AND NON-HOST PLANTS

A. Scala, L. Pazzagli, C. Comparini, A. Santini, S. Tegli, G. Cappugi

Abstract


Cerato-platanin (CP), a previously purified and sequenced protein of about 12.4 kDa, has been studied with regard to the relationship between the fungal growth and the in vitro production by Ceratocystis fimbriata f.sp. platani, the causal agent of plane canker stain. Obtaining CP-specific polyclonal antibodies from rabbit allowed us to prepare a quantitative ELISA which indicated that CP was produced by C. fimbriata f.sp. platani in the first days of in vitro culture. Moreover, for the first time it was demonstrated that a small amount (<2 nmol) of CP elicited the synthesis of phytoalexins such as umbelliferone and glyceollin, in host plane leaves and non-host soybean cotyledons, respectively.

Keywords


Cerato-platanin; elicitor; umbelliferone; glyceollin; ELISA

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DOI: http://dx.doi.org/10.4454/jpp.v86i1.934

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