DETECTION AND MOLECULAR CHARACTERIZATION OF GRAPEVINE FANLEAF VIRUS AND GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 3 IN JORDAN

G.H. Anfoka, W. Shahrour, M.K. Nakhla

Abstract


Grapevine fanleaf virus (GFLV) and Grapevine leafroll-associated virus 3 (GLRaV-3) have been detected by Double-Antibody Sandwich (DAS)-ELISA in six locations where grapevines are commonly grown in Jordan. Using pairs of specific primers, fragments of the RNA-dependent RNA polymerase (RdRp) gene of GLRaV-3 and the coat protein (CP) gene of GFLV were amplified from symptomatic grapevine tissues by Immunocapture- Reverse Transcriptase-Polymerase Chain Reaction (IC-RT-PCR). After cloning and sequencing, sequences of the amplified fragments were deposited in Genbank. Alignment analysis revealed that the amplified CP gene fragment of the Jordanian isolate of GFLV (GFLV-JOR) shared sequence identity between 82 and 88% with 11 GFLV isolates from different parts of the world. High sequence identity (95-99%) was observed between the amplified fragment of RdRp gene of GLRaV-3 from Jordan (GLRaV-3-JOR) and isolates from Brazil, USA, Czech Republic and China.

Keywords


grapevine; DAS-ELISA; IC-RT-PCR; GLRaV-3; GFLV

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DOI: http://dx.doi.org/10.4454/jpp.v86i3.989

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EDIZIONI ETS, Pisa, Italy