FIRST REPORT OF AUSTRALIAN GRAPEVINE VIROID FROM THE MEDITERRANEAN REGION

A. Elleuch, M. Marrakchi, J.P. Perreault, H. Fakhfakh
doi: 10.4454/jpp.v85i1.1009
Abstract:
Grapevines can be infected by five different viroids, of which Australian grapevine viroid (AGVd) is the least studied. Here we report the use of RT-PCR for the detection of this viroid from infected grapevines in Tunisia. RT-PCR results were confirmed by cDNA sequencing. This is the first report of AGVd from the Mediterranean region. The data presented indicate a wider geographical distribution of this viroid than previously reported and record its occurrence in Africa. The molecular variability of AGVd from two different cultivars, Carignan and Syrah, was studied by restriction analyses and by cDNA sequencing. Sequence variability was not clustered in any specific domain or region of the genome.
Vol. 85, N° 1 (2003)
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