CHARACTERIZATION OF A SUBGROUP II ISOLATE OF CUCUMBER MOSAIC VIRUS FROM BITTER GOURD IN CHINA
L. Zhu, Z.P. Xu, C.Y. Fei, D.H. Xi
doi: 10.4454/jpp.v99i2.3870
Abstract:
Diseased bitter gourd plants with severe leaf rolling symptoms were observed in Sichuan province (southwest China). Leaf dips-prepared from symptomatic leaves contained particles ca. 30 nm in diameter. The virus recovered from all samples was identified as Cucumber mosaic virus (CMV) by Western blotting, RT-PCR and sequencing of amplicons. All obtained sequences were identical, and the virus isolate was designated SC-J1. The complete nucleotide sequence of the CP (GenBank accession No. KT932936) of the isolate shared 99% identity to the corresponding regions of strain Hnt (CMV II). Phylogenetic analysis based on the nucleic acid sequence of the CP shows that SC-J1 belongs to CMV subgroup II. To our knowledge, this is the first report of CMV II infecting bitter gourd in China.
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