CHERRY LEAFROLL VIRUS IN JUGLANS REGIA IN THE LAKE VAN BASIN OF TURKEY
M.O. Ozturk, H.M. Sipahioglu, M. Ocak, M. Usta
doi: 10.4454/jpp.v90i1.594
Abstract:
Walnut orchards of the Lake Van basin (Turkey) were surveyed from June to October 2006 to determine the incidence of viral infections. ELISA and RT-PCR were used to investigate the presence of Cherry leaf roll virus (CLRV) and Plum pox virus (PPV), testing a total of 870 samples collected from traditional seed-grown plantations. Whereas no PPV was detected in any of the samples, CLRV was found for the first time in the surveyed locations with an average incidence of 12.9%. Two viral isolates from Edremit were mechanically transmitted to Chenopodium amaranticolor in which they caused local chlorotic spots followed by development of small leaves and apical necrosis. A 366 bp DNA fragment was amplified by RT-PCR from the 3’ noncoding region of RNA-2 of both viral isolates and sequenced. Isolate Edremit-2 was 93-98% identical to the comparable sequences of other isolates for which information is available, whereas isolate Edremit-1 had a lower sequence identity (53-46%). The size of the coat protein subunits of both viral isolates was 52.4 kDa as determined by electrophoresis.
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