MONITORING AMERICAN PLUM LINE PATTERN VIRUS IN PLUM BY ELISA AND DOTBLOT HYBRIDISATION THROUGHOUT THE YEAR
M. Al Rwahnih, A. Myrta, M.C. Herranz, V. Pallás
doi: 10.4454/jpp.v86i2.953
Abstract:
American plum line pattern virus (APLPV) was monitored monthly throughout a year in a Japanese plum tree by using ELISA and dot-blot hybridisation. Leaf samples were tested from March to November and dormant buds were tested from December to February. Flowers, cortex and fruits, when available, were also tested. For leaf samples, detection by ELISA and hybridisation was reliable from March to May. For dormant bud samples, detection was reliable from December to February. Detection levels decreased from June to August, and were unsatisfactory from September to November. During this latter period, molecular hybridisation was more sensitive than ELISA. Leaves were better sources of APLPV than flowers and cortical tissues in spring, whereas mature fruits were better sources than leaves in summer.
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