DETECTION OF PHYTOPLASMAS ASSOCIATED WITH GRAPEVINE FLAVESCENCE DORÉE DISEASE USING REALTIME PCR

P.A. Bianco, P. Casati, N. Marziliano
doi: 10.4454/jpp.v86i3.996
Abstract:
Flavescence dorée (FD) of grapevine is a serious disease caused by phytoplasmas. Currently available protocols for phytoplasma detection in grapevine are complex and time consuming. This work reports the results of a real-time PCR assay (TaqMan®) for detecting phytoplasmas in grapevines affected by FD. We obtained specific detection in samples from seven FD-affected grapevines. This method gave no signal with phytoplasmas associated with another grapevine disease (Bois noir) or with eleven reference phytoplasma strains grown in periwinkle. We propose Real-time PCR as an alternative and rapid method for the detection of phytoplasmas in grapevine.
Vol. 86, N° 3 (2004)
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