Archivio Journal of Plant Pathology

Abstract:

Several viruses from the genus Allexivirus, family Alphaflexiviridae, infect garlic and other species of Al- lium. They commonly occur in multiple infections and are thought to be mite-transmitted (Lu et al., 2008). A survey for viruses was done in garlic plants showing mosaic, de- formation, and yellow stripes in 13 fields located in differ- ent regions of Poland during the 2011-2012 growing season. Total RNA was extracted from bulb tissue using a Spec- trum plant total RNA kit (Sigma-Aldrich, USA) and tested for Garlic virus D (GarV-D), Garlic virus E (GarV-E), and Garlic virus X (GarV-X) by reverse RT-PCR using the tran- scriptor one-step RT-PCR kit (Roche, USA) and primers: (i) DCPF/DCPR 5’- AAGGAGCTACACCGAAGGAC-3’ and 5’-TAAAGTCGTGTGGATGCATCAGA-3’; (ii) EF2/ ER2 5’-TTGCTAGACCACCTCAGTATTGAGAA-3’ and 5’-TATTGGGCGTACATCGGTGACTGT-3’; (iii) XF/XR 5’-GCGGTAATATCTGACACGCTCCA-3’ and 5’-ACGTTAGCTTCACTGGGGTAGAATAT-3’, respec- tively. Products of the expected size (456 bp for GarV-D, 458 bp for GarV-E, and 386 bp for GarV-X) were amplified from 27 (87%), 16 (52%) and 26 (84%) samples, respectively. Twelve samples were infected by the three viruses and 12 by GarV-D and GarV-X. To our knowledge, this is the first report of GarV-D, GarV-E and GarV-X in garlic in Poland.