Archivio Journal of Plant Pathology

Abstract:

The first complete nucleotide sequence of a Grapevine virus B (GVB-QMWH: GVB-Qimiaowuhe) isolate from China is reported. The genome organization of GVB- QMWH is similar to that of previously described GVB isolates. However, the 7,610 nt genomic RNA is the largest of the GVB genomes reported to date, including a 10 nt insertion in the 3! UTR. Moreover, the sizes of open reading frame (ORF) 1 and 2 did not correspond to those found in the other previously published GVB isolates (EF583906, GU733707, JX513897 and X75448). ORF1 of GVB-QMWH was 6 nt longer than that of EF583906, GU733707 and JX513897, and 12 nt longer than that of X75448. ORF2 was 21 nt smaller than that of EF583906, GU733707 and JX513897, and 27 nt smaller than that of X75448. The isolate was found to be less than 80% similar to other reported GVB variants, and phylogenetic analysis based on the complete nucleotide sequence suggested that GVB- QMWH is distantly related to other isolates.