Archivio Journal of Plant Pathology

Abstract:

Tobacco vein banding mosaic virus (TVBMV) is a distinct potyvirus infecting solanaceous plants with increasing importance for tobacco and other crops in mainland China (Zhang et al., 2011). During an investigation on the host range of TVBMV, we collected 180 potato (Solanum tuberosum) samples showing mild vein banding and mosaic symptoms from Shandong Province. Six samples collected in Linyi reacted positively in PTA-ELISA and Western blot assay with antiserum against TVBMV coat protein. Total RNA was extracted from leaves of a potato sample designated as FX using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and reverse transcribed with M-MLV using primer complementary to the 3’-end of TVBMV coat protein ORF (5’-CTACACGCCACTCACACCAAG-3’). The CP ORF of the TVBMV isolate FX was amplified in PCR with the reverse primer mentioned above and the forward primer identical to the 5’-terminal sequence of TVBMV CP ORF (5’-AATGACGAACAGACAGTTGATGCTG-3’). The PCR fragment was cloned into pGEM-T vector and sequenced. The Blast results indicated that the 816 nt-long CP ORF of the isolate FX (GenBank accession No. DQ917752) showed 99.88% identity with that of TVBMV isolate YND (EF219408) from Yunnan province (Yu et al., 2007). To the best of our knowledge, this is the first report of TVBMV on potato in China. As its importance for the industry is yet to be studied, measures should be taken to avoid the infection of TVBMV in production of virus-free seed potatoes.