A METHOD OF ACQUIRING INOCULUM OF RICE BLACK STREAKED DWARF VIRUS FROM WHEAT DARKGREEN
X.Y. Cao, L.L. Du, J.Y. Lv, X.F. Ma, X. Wang, Y.J. Fan, Y.J. Zhou, T. Zhou
doi: 10.4454/jpp.v99i1.3816
Abstract:
Maize rough dwarf disease (MRDD) is one of the main viral diseases of maize, and the cause of great yield losses; one of its causal agents is Rice black-streaked dwarf virus (RBSDV). Here, we developed a method for transmitting RBSDV to maize by Laodelphax striatellus Fallén, the small brown planthopper (SBPH) from wheat plants affected by wheat dark-green dwarf disease (WDGDD). The SBPH transmitted RBSDV more efficiently when it was acquired from wheat than from maize, with an efficiency of 36.7% and 12.7%, respectively. We also found that when the same number of vectors was used, transmission efficiency was similar after feeding on either WDGDD- or MRDD-infected plants. These results indicate that the method is efficient and viable, and is expected to facilitate the identification of maize cultivars that are resistant to RBSDV.
Indietro
