Archivio Journal of Plant Pathology

Abstract:

Grapevine Syrah virus-1 (GSyV-1) is a member of the genus Marafivirus, family Tymoviridae. This virus, originally reported from the USA, has now been reported from several Europan countries (Glasa et al., 2015) and more recently from Chile, Brazil and South Africa. Dormant canes of grapevine cvs. Syrah, Palieri and Antep karası were collected in Turkey and screened by RT-PCR for the presence of GSyV-1, Grapevine Pinot gris virus (GPGV), Grapevine virus A (GVA) and Grapevine rupestris stem pitting-associated virus (GRSPaV). Three of the 302 samples (cvs. Syrah and Antep karası) were found to be infected with GSyV-1 using two different primers, GSyV-1Det-F/Det-R (the putative methyltransferase gene) (Al Rwahnih et al., 2009) and GVQCP-F/R (partial CP gene) (Sabanadzovic et al., 2009). GSyV-1 was detected using both primers in cv. Syrah, whereas it was detected by only GSyV-1Det-F/Det-R in cv. Antep karası. All PCR products were sequenced and the sequences were deposited in GenBank. Comparison of the partial methyltransferase gene sequences (KY558548 and KY558550) showed 90-98% nucleotide identity to different GSyV-1 sequences available in GenBank, while the partial CP gene sequence (KY558549) revealed 89-97% nucleotide identity to the database sequences. Nucleotide sequences of both genes shared the highest sequence identity at the nucleotide level (97-98%) with different European GSyV-1 isolates. Among the GSyV-1-positive samples, two were also infected by GPGV, GVA and GRSPaV. No particular virus-like symptoms were observed on GSyV-1-infected grapevines. To our knowledge this is the first report of GSyV-1 in Turkey.